Abstract: Objective :To study the important role of c-FLIP, which structurally simulates theapoptotic effector caspase-8, in cell apoptosis and to provide a new methodology or protocol of antisense oligonucleotides (ASODN) of the gene for treatment of human gastric cancer. Methods :Reversetranscriptase polymerise chain reaction and Western Blot were used to detect c-FLIPs (cellular FLI sha.and cellular FLIP,omRNA and protein in both Hela and BGC823 cell lines. Whether c-Flip is functionally significant for the cell line or not was determined and 5' FAM-conjugated antisense oligonucleotides (ASODN) wire created complementary to a sequence that included the start site of thec-Flip open reading frame. C- FLIP ASODN was introduced into cell line BGC823 with lipofectamine 2000 as the vector, and its effect and mechanism wire investigated by M7rUNEL, FACs and Western blot assay. Results :The encoding mRNA and protein of c-FLIPS and c-FLIPS can be detected in both cell lines. MTT assay revealed that liposome mediated antisense oligonucleotides po-AS) c an significantly inhibit BGC823 cell proliferation, with the effect apparently related with the concentration;TUNEL staining was positive, specific apoptotic peak can be detected before G, peak by FACs, and Western Blot analysis revealed that the protein expression of c-FLIPS and c-FLIPS decreased significandy after the cell was treated with ascending concentration ASODN. Conclusions :The c-FLIP Hela and BGC823 cell line. Liposome-mediated ASODN down-regu-lates the c-FLIPS protein expression and initiates the apoptosis. Researches on antisense drugs targeting on c-FLIP gene wound provide the new methods and protocols for treatment of gastric carcinoma.